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Admission cell 2024-2025

Dissecting Microscope

Standard Operating Procedure (SOP) for Dissecting Microscope

1. Purpose:

To provide a standardized method for operating and maintaining a dissecting microscope to ensure accurate and efficient observations of larger or three-dimensional specimens.

2. Scope:

This SOP applies to all personnel involved in the use of the dissecting microscope in the laboratory for detailed examination and dissection of specimens.

3. Responsibility:

  • Laboratory Technicians: Operate the dissecting microscope as per the SOP.
  • Lab Manager: Ensure the SOP is followed and updated as needed.

4. Equipment and Materials:

  • Dissecting Microscope
  • Specimen to be examined or dissected
  • Light source (if applicable)
  • Dissection tools (e.g., forceps, scalpels)
  • Personal Protective Equipment (PPE): Lab coat, gloves, safety goggles
  • Lens cleaning paper or microfiber cloth

5. Procedure:

5.1 Pre-Operation:

  1. Ensure the dissecting microscope is placed on a stable, flat surface free from vibrations and disturbances.
  2. Check the microscope for cleanliness and ensure the lenses and stage are free of dust and residues.
  3. Ensure the light source is functioning correctly and adjust it for optimal illumination.
  4. Wear appropriate PPE before starting the procedure.

5.2 Setting Up the Microscope:

  1. Adjust the microscope to a comfortable working height and position.
  2. Position the specimen on the microscope’s stage. Use the stage clips or platform to secure the specimen if necessary.

5.3 Adjusting the Microscope:

  1. Use the focus knobs to adjust the distance between the objective lenses and the specimen. This will bring the specimen into clear view.
  2. Adjust the light source to ensure even and adequate illumination of the specimen.
  3. If the microscope has adjustable ocular lenses, adjust them to suit your vision.

5.4 Observing the Specimen:

  1. Look through the eyepieces and adjust the focus to obtain a clear image of the specimen.
  2. Use the microscope’s zoom controls to change magnification and examine different details of the specimen.
  3. Manipulate the specimen using dissection tools as needed for detailed examination.

5.5 Post-Operation:

  1. After completing the observation or dissection, remove the specimen from the stage.
  2. Clean any residues or debris from the stage and surrounding area.
  3. Turn off the light source if applicable.
  4. Cover the microscope to protect it from dust and debris.

5.6 Maintenance:

  1. Regularly clean the lenses and other optical components using lens cleaning paper or a microfiber cloth.
  2. Inspect the microscope for any signs of wear or damage and address any issues promptly.
  3. Perform routine checks on the light source and replace bulbs or batteries as needed.
  4. Record maintenance activities in the equipment logbook.

6. Safety Measures:

  1. Always wear appropriate PPE when handling specimens and using the microscope.
  1. Handle dissection tools and specimens with care to avoid injury and contamination.
  2. Ensure proper ventilation in the area where the dissecting microscope is used.
  3. Dispose of used specimens and waste materials according to the laboratory’s waste disposal guidelines.

7. Documentation:

  1. Record the date, time, and purpose of each use in the dissecting microscope logbook.
  2. Note any deviations from the SOP and report them to the lab manager.

8. References:

  • Manufacturer’s Manual for the Dissecting Microscope
  • Laboratory Safety Guidelines
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