Trinocular Microscope

Standard Operating Procedure (SOP) for Trinocular Microscope

1. Purpose:

To provide a standardized method for operating and maintaining the trinocular microscope to ensure accurate and reliable observations of specimens, and to utilize the third ocular for photographic or video documentation.

2. Scope:

This SOP applies to all personnel involved in the use of the trinocular microscope in the laboratory.

3. Responsibility:

• Laboratory Technicians: Operate the trinocular microscope as per the SOP.
• Lab Manager: Ensure the SOP is followed and updated as needed.

4. Equipment and Materials:

• Trinocular Microscope
• Prepared slides or specimens
• Lens cleaning paper or microfiber cloth
• Camera or video system
• Personal Protective Equipment (PPE): Lab coat, gloves, safety goggles

5. Procedure:

5.1 Pre-Operation:

1. Ensure the trinocular microscope is placed on a stable, flat surface free from vibrations and disturbances.
2. Check the microscope for cleanliness, and ensure the lenses, stage, and other components are free of dust and residues.
3. Adjust the microscope to a comfortable working height and position.
4. Ensure the light source (if applicable) is functioning correctly and adjust it for optimal illumination.
5. Wear appropriate PPE before starting the procedure.

5.2 Setting Up the Microscope:

1. Place the prepared slide or specimen on the stage and secure it with the stage clips.
2. Select the lowest magnification objective lens and ensure it is in position over the specimen.
3. Adjust the light source or mirror to provide adequate and even illumination.

5.3 Focusing the Microscope:

1. Look through the eyepieces and adjust the interpupillary distance to match the distance between your eyes.
2. Use the coarse adjustment knob to bring the specimen into rough focus.
3. Switch to the fine adjustment knob to achieve a sharp and clear image.
4. If using higher magnification, rotate the nosepiece to the desired objective lens and refocus using the fine adjustment knob.

5.4 Observing and Documenting the Specimen:

1. Observe the specimen through the binocular eyepieces, adjusting focus and illumination as needed.
2. For documentation, align the camera or video system with the trinocular port.
3. Adjust the camera settings (focus, exposure, etc.) for clear and accurate images or videos.
4. Capture images or record video of the specimen as required.

5.5 Post-Operation:

1. After observing and documenting the specimen, rotate the nosepiece to the lowest magnification lens.
2. Remove the slide from the stage and clean any immersion oil or residues from the lenses and stage using lens cleaning paper or a microfiber cloth.
3. Turn off the light source if applicable.
4. Disconnect and store the camera or video system if used.
5. Cover the microscope to protect it from dust and debris.

5.6 Maintenance:

1. Regularly clean the lenses and optical components using lens cleaning paper or a microfiber cloth.
2. Inspect the microscope for signs of wear or damage and address any issues promptly.
3. Perform routine checks on the light source and replace bulbs or batteries as needed.
4. Ensure the camera or video system is functioning correctly and perform routine maintenance as specified by the manufacturer.
5. Record maintenance activities in the equipment logbook.

6. Safety Measures:

1. Always wear appropriate PPE when handling specimens and using the microscope.
2. Handle slides, specimens, and camera equipment with care to avoid damage and contamination.
3. Ensure proper ventilation in the area where the microscope is used.
4. Dispose of used slides and specimens according to the laboratory’s waste disposal guidelines.

7. Documentation:

1. Record the date, time, and purpose of each use in the trinocular microscope logbook.
2. Note any deviations from the SOP and report them to the lab manager.

8. References:

• Manufacturer’s Manual for the Trinocular Microscope
• Laboratory Safety Guidelines