Menu
Admission cell 2024-2025

UV Visualizing Chamber

Standard Operating Procedure (SOP) for UV Visualizing Chamber

1. Purpose:

To provide a standardized method for operating and maintaining the UV visualizing chamber for detecting and analyzing UV-active substances or compounds.

2. Scope:

This SOP applies to all personnel involved in using the UV visualizing chamber in the laboratory.

3. Responsibility:

  • Laboratory Technicians: Operate the UV visualizing chamber according to the SOP.
  • Lab Manager: Ensure the SOP is followed and updated as needed.

4. Equipment and Materials:

  • UV Visualizing Chamber
  • UV light source (UV lamp)
  • Sample plates (e.g., TLC plates)
  • Personal Protective Equipment (PPE): Lab coat, gloves, UV-blocking safety goggles

5. Procedure:

5.1 Pre-Operation:

  1. Ensure the UV visualizing chamber is clean and free from any contaminants.
  2. Check that the UV lamp is functioning correctly and replace it if necessary.
  3. Verify that the chamber is placed on a stable, flat surface.
  4. Wear appropriate PPE before starting the procedure.

5.2 Preparing the Chamber:

  1. Open the UV visualizing chamber and ensure that the UV lamp is turned off before placing any samples inside.
  2. Arrange the sample plates (e.g., TLC plates) on the stage or platform inside the chamber.
  3. Close the chamber securely to ensure safety and prevent UV exposure.

5.3 Visualizing Samples:

  1. Turn on the UV lamp inside the visualizing chamber.
  2. Allow the lamp to warm up
  3. Observe the samples through the viewing window of the chamber. UV-active substances will typically fluoresce or appear as spots or bands under UV light.
  4. Use appropriate filters or wavelengths as needed based on the nature of the samples and the required analysis.

5.4 Documenting Observations:

  1. Record the observations, including the appearance of spots or bands, their location, and intensity.
  2. If required, take photographs of the visualized samples using a suitable camera or imaging system for documentation.

5.5 Post-Operation:

  1. Turn off the UV lamp before opening the chamber.
  2. Remove the sample plates from the chamber once observations are complete.
  3. Clean the interior of the chamber with a mild detergent and water if needed. Rinse thoroughly to remove any residues.
  4. Close and secure the chamber for storage.

5.6 Maintenance:

  1. Regularly inspect the UV visualizing chamber for signs of wear, damage, or malfunction.
  2. Replace the UV lamp according to the manufacturer’s recommendations or if it becomes dim or fails.
  3. Perform routine cleaning and maintenance as specified by the manufacturer.
  4. Record maintenance activities in the equipment logbook.

6. Safety Measures:

  1. Always wear appropriate PPE, including UV-blocking safety goggles, when operating the UV visualizing chamber.
  2. Avoid direct exposure to UV light to prevent potential skin and eye damage.
  3. Ensure the chamber is properly closed and secured during operation to prevent UV leakage.
  4. Follow all laboratory safety guidelines and protocols when handling UV-sensitive equipment and materials.

7. Documentation:

  1. Record the date, time, and purpose of each use of the UV visualizing chamber in the lab notebook or equipment logbook.
  2. Note any deviations from the SOP and report them to the lab manager.
  3. Document observations and any photographic evidence as required.

8. References:

  • Manufacturer’s Manual for the UV Visualizing Chamber
  • Laboratory Safety Guidelines

Loading

Hindi »